Effect of Vitrification in Open Pulled Straws on Survival of Bovine Embryos from Superovulated Cows
نویسندگان
چکیده
منابع مشابه
The effect of vitrification in open pulled straws on pregnancy rates after transfer of in vivo produced bovine embryos
The aim of this study was to compare pregnancy rates after transfer of in vivo produced embryos cryopreserved using open pulled straw (OPS) vitrification (Group V) or conventional freezing method as a control (Group C). Bovine embryos (Day6.5–7.5) collected from superovulated cows were classified according to developmental stages and morphological qualities (Grade 1 and 2) before cryopreservati...
متن کاملAseptic technology of vitrification of human pronuclear oocytes using open-pulled straws.
BACKGROUND The aim of this study was to compare the viability of human pronuclear oocytes subjected to vitrification using cooling by direct submerging of open-pulled straws in liquid nitrogen versus vitrification by cooling of open-pulled straws located inside a closed 0.5 ml straw (aseptic system). METHODS Two- and three-pronuclei stage oocytes (n=114) were cryopreserved in super-open-pulle...
متن کاملOpen pulled straws for vitrification of mature mouse oocytes preserve patterns of meiotic spindles and chromosomes better than conventional straws.
Vitrification of oocytes has been applied recently for humans, but remains elusive. The microtubules of oocytes are vulnerable to cryoprotectants and thermal changes. Using mouse oocytes, the effects of vitrification in open pulled straws (OPS) were investigated on survival, the meiotic spindle, and chromosomes and compared with conventional straws. Mature oocytes were allocated to four groups ...
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ژورنال
عنوان ژورنال: Acta Veterinaria Brno
سال: 2002
ISSN: 0001-7213,1801-7576
DOI: 10.2754/avb200271010093